Many studies have been increasingly applied to analyze medicinal plants which proved to be an excellent method for the analysis of non-polar compounds and volatile essential oil

Many studies have been increasingly applied to analyze medicinal plants which proved to be an excellent method for the analysis of non-polar compounds and volatile essential oil, fatty acids, lipids and alkaloids. GC-MS is widely used in pharmaceutical industries and is a complementary part of research associated with medicinal chemistry (synthesis and characterization of compounds), pharmaceutical analysis (stability testing, impurity profiling), pharmacognosy, pharmaceutical process control and pharmaceutical biotechnology.
The GC-MS is consist of two components: the gas chromatograph and the mass spectrometer. The gas chromatograph utilizes a capillary column which depends on the column’s dimensions (length, diameter, film thickness) as well as the phase properties (e.g. 5% phenyl polysiloxane). The separation of the different molecules within a mixture depend on the chemical properties between these molecules and on their relative affinity for the stationary phase of the column during the pass of the sample along the column. The molecules are retained and then elute from the column at different times (called the retention time), downstream the mass spectrometer capture, ionize, accelerate, deflect, and detect the ionized molecules separately by breaking each molecule into ionized fragments and detecting these fragments using their mass-to-charge ratio